The vapor phase of liquid nitrogen LN2 (-150°C)is typically used to store PBMC’s. It is quite possible that Cryopreserved samples could possibly be frequently exposed to GTTW during retrieval of specimens, particularly from large active cryogenic repository collections. We have determined the rate and the degree that frozen samples will warm when removed from a liquid nitrogen freezer. A freezer rack enclosing mock samples was removed and laid on the top of an open liquid nitrogen freezer. With our computerized freezer monitoring system, we demonstrated that the mock samples' temperature had increased by between 14°C to 55°C, after just 5 minutes in this position, which equaled an average temperature of -114°C. This rise was well above the GTTW (-132°C).
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